ACID FAST BACILLI- AFB

200 Rs

MRP

Discounted Price:

100 Rs

Pre-test preparation or requirements

• At least two sample should be collected including one early morning sample.

Normal range

AFB GRADING

EXAMINATION              RESULT          GRADING      NO OF FIELD

1-10 AFB/OIF                   POSITIVE                2+                        100

1-10 AFB/OIF                   POSITIVE                2+                        100

10-99 AFB/100 OIF      POSITIVE                 1+                        100

1-9 AFB/100 OIF            SCANTY     RECORD EXACT NO 100

NO OF AFB/100 OIF    NEGATIVE                --                          100

Significance of test

Acid-fast bacteria/ acid-fast bacilli/ AFB, is a group of bacteria having the characteristic of acid fastness. Acid fastness is a physical property of bacteria that gives the ability to resist decolorization by acids during staining procedure.
Bacteria displaying acid fastness include:

Genus Mycobacterium – M. leprae, M. tuberculosis, M. smegmatis, M. Avium complex, M. kansasii.
Genus Nocardia – N. brasiliensis, N. cyriacigeorgica, N. farcinica, and N. nova.

There are 4 main ways of collecting sputum.

Coughing is the most common and widely used method for sputum collection.
Sputum induction is used for patients who are unable to expectorate sputum by just coughing alone. Deep productive coughing may be induced by inhalation of aerosolized warm hypertonic saline (3 to 5%).
Bronchoscopy is an invasive procedure used to visualize the respiratory passages of the lungs. This procedure may be used to extract sputum via bronchial washing, brushing, and/or biopsying.
Gastric aspiration can be used to obtain sputum previously expectorated and swallowed by the patient. This procedure is particularly useful in children.

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