ANTI HEPATITIS C VIRUS- HCV
Pre-test preparation or requirements
• Sample can be drawn at any time. No fasting or special preparation required.
• Sample should taken in plain vial.
Test specimen with absorbance value less than cutoff value is non reactive and may be considered as negative for Anti HCV.
Significance of test
Test specimen with absorbance value more than or equal to cutoff value is reactive for Anti HCV.
Hepatitis C Virus (HCV) has been identified as the main etiological agent of Non A and Non B hepatitis accounting for 80-90 cases of transfusion hepatitis cases. HCV is transmitted primarily through par enteral routes. HCV antibodies have been found in patients with acute or chronic forms of Hepatitis C and in many asymptomatic donors.
The 3rd generation HCV Microlisa is based on a highly sensitive technique, Enzyme Linked Immunosorbent Assay which detects antibodies against HCV in human serum and plasma. The HCV proteins are present in serum at levels well below the limits of detection. Thus immunodiagnosis of HCV infection is based on detection of host generated antibodies (anti-HCVs) to viral proteins. The 3rd generation HCV Microlisa utilizes a combination of antigen with the sequence of both HCV structural and non-structural antigen i. e. CORE, E1, E2, NS5. It has an obvious advantage over the available 2nd generation and 1st generation ELISA with improved sensitivity and specificity.