Pre-test preparation or requirements
• Sample can be drawn at any time. No fasting or special preparation required.
• Sample should taken in plain vial.
Test specimen with absorbance value less than cutoff value is non reactive and may be considered as negative for HBsAg.
Test specimen with absorbance value more than or equal to cutoff value is reactive for HBsAg.
Significance of test
The hepatitis B Virus causes viral hepatitis.. 1-5% infected people act as chronic carriers of Hepatitis B virus.. Major part of the chronic carriers secrete hepatitis B surface antigen (HBsAg) into the blood and other secretions of the body like saliva and vaginal fluid. These chronic carriers are potentially infectious to other seronegative people. Hepatitis B Virus belongs to a family of enveloped DNA virus, the Hepadnavirus. Related viruses in this group cause chronic hepatitis. HBsAg has been accepted as a universal and the most reliable seromarker in case of acute HBV infection due to its appearance almost 2-4 weeks before the ALT level becomes abnormal and 3-5 weeks before the onset of symptoms or jaundice as an early detection system for hepatitis infection. In most cases of HBV infection, the incubation period varies from 40 days to 6 months.